EuroFlow LST 2026
Resources
Detailed information
EuroFlow SOPs and relevant documents
EuroFlow SOP for sample preparation. Version 1.7 - 1 November 2023
EuroFlow SOP for instrument setup and compensation for DxFlex. Version 2.0 – February 2025
EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto II. Version 2.0 – February 2025
EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels. Version 2.0 – June 2025
EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.3 – February 2025
EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 – October 2019
Composition and technical info of EuroFlow panels for hematological malignancies. Version 1.12 – February 2025
List of reference and alternative antibodies for hematological malignancies panels. Version 1.14 – February 2025
EuroFlow target MFI values for Rainbow beads. Version 1.20 - April 2025
The above documents can be accessed after creating a EuroFlow account and upon login at https://app.euroflow.org/login.
Downloads for BD Lyric (if applicable):
Reagent files (zip) (download and extract) – June 2025
Assay files (zip) (download and extract) – June 2025
Label file (xml)
Videos
Educational video: EuroFlow SOP for instrument setup and compensation (for BD LSR II and BD FACS Canto)
euroflow.org
Publications
Kalina et al., Quality assessment program for EuroFlow protocols: summary results of four-year (2010-2013) quality assurance rounds. Cytometry A. 2015 Feb;87(2):145-56.
pubmed.ncbi.nlm.nih.gov
Kalina et al., Frequent issues and lessons learned from EuroFlow QA. J Immunol Methods. 2019 Dec;475:112520.
pubmed.ncbi.nlm.nih.gov
Flores-Montero et al., EuroFlow Lymphoid Screening Tube (LST) data base for automated identification of blood lymphocyte subsets. J Immunol Methods. 2019 Dec;475:112662.
pubmed.ncbi.nlm.nih.gov
van Dongen et al., EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012 Sep;26(9):1908-75.
pubmed.ncbi.nlm.nih.gov